Nucleotide sequence encoding genes for isoleucine- and alanine-accepting tRNA from Pseudomonas aeruginosa.

SEQUENCE AND ITS ORIGIN: Fragments of Pseudomonas aeruginosa (strain K, ATCC 25102) DNA encoding two tRNA genes have been seguenced as part of a project directed towards sequencing a complete ribosomal RNA operon from this organism. Clones from a BamHl library of P. aeruginosa DNA ligated into plasmid pOC9 were isolated by hybridisation with P. aeruginosa 16S and 23S ribosomal RNA molecules that had been labelled with P at the 5'-terminus. A 4.5 kb insert from one of these clones was subfragmented and cloned into Ml3mp8 and Ml3mp9 sequencing vectors to enable sequencing by the chain termination method (1). Both DNA strands were secuenced at least twice.